Part:BBa_K3714004

Improved theophylline biosensor

An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (BBa_J119449).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

By introducing mutations in loop Ⅰ and loop Ⅱ[1], we achieved an aptazyme with higher responsing fold and less leakage (Fig.1).

Figure 1 | Improvement of an existing theophylline aptazyme. a.Cleavage properties of two aptazymes under different theophylline concentration. The sequences was synthesized and transcribed in vitro. The number above each lane means dilution fold of saturated theophylline aqueous solution at 25℃. b. A diagram showing the intensity percentage of the cleaved band of each lane in a. Intensity was measured by ImageJ.