RNA
7CG

Part:BBa_K3714004

Designed by: Yuxin Lu   Group: iGEM21_SJTU-BioX-Shanghai   (2021-09-30)
Revision as of 02:32, 21 October 2021 by Grus (Talk | contribs)


Improved theophylline biosensor

An improved aptazyme responsing to theophylline, which means its self-cleaving activity can be inhibited by adding theophylline. This aptazyme shows higher sensibility than an existing part (BBa_J119449).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


K3714004.png
Figure.1 | Improvement of an existing theophylline aptazyme. a.Cleavage properties of two aptazymes under different theophylline concentration. The number above each lane means dilution fold of saturated theophylline aqueous solution at 25℃. b. A diagram showing the intensity percentage of the cleaved band of each lane in a. Intensity was measured by ImageJ.


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Categories
Parameters
None