RNA
gard-337

Part:BBa_K3714000

Designed by: Yuxin Lu   Group: iGEM21_SJTU-BioX-Shanghai   (2021-08-30)
Revision as of 17:32, 20 October 2021 by Grus (Talk | contribs)


Gardiquimod biosensor 337

The template of a newly found self-cleaving aptazyme, which can keep itself intact with gardiquimod present, and catalyze the self-cleavage reaction in the absence of gardiquimod. This part could be used as a switch for gene expression controlled by gardiquimod.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


K3714000.png
Figure.1 | Verification of biosensor gard-337. An in vitro transcription system was employed to test if this biosensor can response gardiquimod signal. The product was run on a denatured PAGE.
[edit]
Categories
//rna/aptazyme
Parameters
efficiency10 μM gardiquimod inhibits 94.4% cleavage[1]