Part:BBa_K3734009

INSR

INSR

Insulin receptor  (INSR)is a membrane protein that bid to insulin, feel insullin concentration and activate the downstream MAPK phosphorylation pathway.We have designed a carrier CMV-INSR-EF1A-ZsGreen containing INSR to express INSR, and its expression can be characterized by the green fluorescent protein ZsGreen. Experiments have proved that INSR is successfully expressed in our engineering cells.

1.1 Method

Observation of green fluorescent protein ZsGreen under laser confocal microscope.

1.2 Result

Fig.1 Electrophoretic diagram of INSR PCR product

INSR is successfully expressed and working in cell 293T

Fig.2 Green fluorescence of INSR expression

2.Caution

Because the INSR sequence is very long, it is difficult for DH5α receptor cells containing recombinant enzymes to meet the requirements during cloning, and receptor cells of recombinant enzymes may need to be removed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 551
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2055
  Illegal BglII site found at 8438
  Illegal BamHI site found at 2401
  Illegal XhoI site found at 809
  Illegal XhoI site found at 2566
  Illegal XhoI site found at 3579
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 537
  Illegal NgoMIV site found at 2639
  Illegal AgeI site found at 7134
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2267
  Illegal BsaI site found at 2577
  Illegal BsaI.rc site found at 502
  Illegal BsaI.rc site found at 744
  Illegal BsaI.rc site found at 1319
  Illegal BsaI.rc site found at 1775
  Illegal BsaI.rc site found at 2097
  Illegal BsaI.rc site found at 3674
  Illegal BsaI.rc site found at 3849
  Illegal SapI site found at 790
  Illegal SapI site found at 8692
  Illegal SapI.rc site found at 3856
  Illegal SapI.rc site found at 9122