Coding

Part:BBa_K3989014

Designed by: Song-Yuan Zhang   Group: iGEM21_UZurich   (2021-10-01)
Revision as of 10:25, 20 October 2021 by ZhangSongyuan (Talk | contribs)


double cellulose binding domain fused with ClyA for OMV display In this construct, outer membrane-associated protein ClyA (Part:BBa_K811000) is used to display the double cellulose binding domain (dCBD, Part:BBa_K3989023) to the surface of bacterial outermembrane vesicles (OMVs) to trigger plant immune responses. To make elf18 better recognized by plant immune receptor FLS2, we put mutiple linkers and a 3×FLAG tag between ClyA and elf18 to minimize the steric hindrance.

Usage and Biology

ClyA-dCBD-SGI.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None