T7

Part:BBa_K4033021

Designed by: Yuhan Zhu   Group: iGEM21_BHSF   (2021-10-01)
Revision as of 13:27, 19 October 2021 by XiXiThea (Talk | contribs)

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T7 promoter

A promoter for the RNA polymerase from T7 bacteriophage. Drives high-level transcription of the downstream sequence of interest.


Biology and Usage

The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Experimental approach



References

[1]Komura R, Aoki W, Motone K, Satomura A, Ueda M. High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding. PLoS ONE. 13(5):e0196905. http://dx.doi.org/10.1371/journal.pone.0196905

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