Part:BBa_K3734002

GIP promoter

GIP is a promoter that can be induced by glucose.

                   GIP promoter
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GIP promoter is anticipated in current literature that it can only be used in several certain types of cells like intestine cells, but we proved that it can function will in 293T cells which are usually used in labs.

1.Pattern diagram

                   <img class="image" src="">

Fig.1 The model diagram of CHREBP-LUC

2.1 Method

We use LUC as report gene, test its real-time luminescence value at 560nm wavelength with specific devices and detect the expression level under different glucose concentration.

2.2 Result

GIP promoter is induced by glucose and the expression level rises along with the raise of glucose concentration.

                   <img class="image" src="">

Fig.2 Changes of LUC expression over time after blue light irradiation with different glucose concentrations

3.Caution

Use medium without glucose when paving in case of the effect caused by glucose residue

Reference：

[1]Anthony T. Cheung,Bama Dayanandan,Jamie T. LewisGlucose-Dependent Insulin Release from Genetically Engineered K Cells[J].Science.2000,290:1959-1962

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 899
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 216