Coding
Z34C

Part:BBa_M10018:Design

Designed by: John Wang   Group: Berkeley BioE140L - S09   (2009-05-07)
Revision as of 00:45, 10 May 2009 by Johnwang (Talk | contribs) (→‎Source)

{˂Z34C domain˃}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

later


Source

Wobble Jw0017F / Jw0018R (133bp, EcoRI/BamHI)
Sub into pBca9145-Bca1144#5 (EcoRI/BamHI, 2057+910, L)
Product is pBca9145-M10018 {Z34C}


Jw0017F Forward construction of Z34C
CCATAgaattcATGagatctTTCAACATGCAGTGCCAGCGTCGTTTCTACGAAGCTCTGCACGACCCGAACCTGAACGAAG
Jw0018R Reverse construction of Z34C
CTGATggatccGCAGTCGTCACGGATAGATTTGATTTTAGCGTTACGCTGTTCTTCGTTCAGGTTCGGGTCG

References