Plasmid

Part:BBa_K4083000

Designed by: Arsen Orazbek   Group: iGEM21_NU_Kazakhstan   (2021-09-08)
Revision as of 12:09, 19 October 2021 by Arsenorazbek (Talk | contribs)


pRGPDuo2 plasmid for P. putida

Duo2 plasmid is engineered by Rahul Gauttam, Aindrila Mukhopadhyay, and Steven W. Singer (2020)[1]. The plasmid was sent to us by Rahul Gauttam who is currently working as a postdoctoral fellow at Berkeley Lab.

About plasmid

This plasmid was modified from the pRG_Duet vector for Corynebacterium glutamicum.

Reference:

[1] Gauttam, R., Mukhopadhyay, A., & Singer, S. W. (2020). Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida. Plasmid, 110. https://doi.org/10.1016/j.plasmid.2020.102514

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3853
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3880
    Illegal BamHI site found at 31
    Illegal XhoI site found at 2175
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6480
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//plasmid
Parameters
None