Composite

Part:BBa_K4083008

Designed by: Arsen Orazbek   Group: iGEM21_NU_Kazakhstan   (2021-10-18)
Revision as of 11:52, 19 October 2021 by Arsenorazbek (Talk | contribs)


RPGDuo2 plasmid with nadE and rhlBA genes

This plasmid consists of four parts: RPGDuo2 plasmid; nadE, rhlA, and rhlB coding sequences (https://parts.igem.org/Part:BBa_K4083000, https://parts.igem.org/Part:BBa_K4083004, https://parts.igem.org/Part:BBa_K4083006, https://parts.igem.org/Part:BBa_K4083007).


By inserting this plasmid, we predicted the effective rhamnolipid production by Pseudomonas putida.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5565
    Illegal BamHI site found at 6125
    Illegal XhoI site found at 1757
    Illegal XhoI site found at 2024
    Illegal XhoI site found at 6301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1949
    Illegal NgoMIV site found at 2019
    Illegal NgoMIV site found at 2240
    Illegal NgoMIV site found at 4400
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None