Composite

Part:BBa_K4040021

Designed by: Tong Yin   Group: iGEM21_NMU_China   (2021-09-27)
Revision as of 11:50, 19 October 2021 by Ytong (Talk | contribs)

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Synthetic Receptor IL6R-TCS-Gal4-KRAB

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1237
    Illegal PstI site found at 1414
    Illegal PstI site found at 1969
    Illegal PstI site found at 2815
    Illegal PstI site found at 2974
    Illegal PstI site found at 3082
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1237
    Illegal PstI site found at 1414
    Illegal PstI site found at 1969
    Illegal PstI site found at 2815
    Illegal PstI site found at 2974
    Illegal PstI site found at 3082
    Illegal NotI site found at 3323
    Illegal NotI site found at 3944
    Illegal NotI site found at 4256
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2692
    Illegal BamHI site found at 3825
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1237
    Illegal PstI site found at 1414
    Illegal PstI site found at 1969
    Illegal PstI site found at 2815
    Illegal PstI site found at 2974
    Illegal PstI site found at 3082
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1237
    Illegal PstI site found at 1414
    Illegal PstI site found at 1969
    Illegal PstI site found at 2815
    Illegal PstI site found at 2974
    Illegal PstI site found at 3082
    Illegal NgoMIV site found at 796
    Illegal NgoMIV site found at 820
    Illegal NgoMIV site found at 892
    Illegal NgoMIV site found at 904
    Illegal NgoMIV site found at 1754
    Illegal NgoMIV site found at 1889
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This part is part of our design of IL-6 complex receptor. We connect TCS (BBa_J18918) behind mil-6R and connect part GAl4_KRAB(BBa_K2446037) downstream of TCS. We used CMV(BBa_I712004) as the promoter.  When IL-6 elevates to a certain level in vivo, MIL-6R forms a hexamer complex with GP130 and IL-6 from BBa_K4040020. TEV protease in BBa_K2446037 is activated. Then it binds to TCS which connects to the downstream of IL-6R and digests the binding site.  Gal4-krab (BBa_K2446037) is released, which modulates the expression of another composite part we designed, BBa_K4040023, and promotes the transformation of macrophages to M2 state by inhibiting the expression of CAR-γ.

Figure. 1The structure of IL6R-TCS-Gal4-KRAB.
Figure. 2The structure and downstream pathway of the composite IL6R.

Experimental result

It can be found in BBa_K4040020.



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