Part:BBa_K3718008
Demonstrative circuit of tool kit part 2
An example product of our toolkit part II after cloning
After the experiments of MRP expression and rescue K.O. E. coli have proven to be successful, we will use caffeine-sensitive MRP as a testing part to model the system. Caffeine will be used to homodimerize the receptors, and is expected to induce the expression of downstream genes. This model will be used to conduct three experiments to confirm the functionality of the tool. By cloning in an antibody VHH gene against caffeine and gfp as a desired gene to be expressed, we expect the platform is activated in the presence of caffeine to express gfp, arcA and iclR, which will result in an increase in growth rate and a visible green color under UV.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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