Composite

Part:BBa_K3745040:Design

Designed by: Jianfei Song   Group: iGEM21_BNDS_China   (2021-09-30)
Revision as of 03:17, 18 October 2021 by Jianfei Song (Talk | contribs) (→‎References)


RhlABC + tags + scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2281
    Illegal BamHI site found at 6640
    Illegal XhoI site found at 6649
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2149
    Illegal NgoMIV site found at 2554
    Illegal NgoMIV site found at 4123
    Illegal NgoMIV site found at 5446
    Illegal NgoMIV site found at 5761
    Illegal NgoMIV site found at 6076
    Illegal NgoMIV site found at 6391
    Illegal AgeI site found at 3166
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5676
    Illegal BsaI site found at 5991
    Illegal BsaI site found at 6306
    Illegal BsaI site found at 6621
    Illegal SapI.rc site found at 4262
    Illegal SapI.rc site found at 5305


Source

This study

References

[1] Dueber, J., Wu, G., Malmirchegini, G. et al. Synthetic protein scaffolds provide modular control over metabolic flux. Nat Biotechnol 27, 753–759 (2009). https://doi.org/10.1038/nbt.1557

[2] Yin X, Liang C, Feng Y, et al. Research progress on synthetic scaffold in metabolic engineering – a review. Chin J Biotech, 2019, 35(3): 363–374.

[3] Du, J., Zhang, A., Hao, J., & Wang, J. (2017). Biosynthesis of di-rhamnolipids and variations of congeners composition in genetically-engineered Escherichia coli. Biotechnology Letters, 39(7), 1041–1048. https://doi.org/10.1007/s10529-017-2333-2

[4] Gong ZJ, Peng YF, Zhang YT, et al. Construction and optimization of Escherichia coli for producing rhamnolipid biosurfactant. Chin J Biotech, 2015, 31(7): 1050–1062.