Translational_Unit

Part:BBa_K3745034:Design

Designed by: Jianfei Song   Group: iGEM21_BNDS_China   (2021-09-30)
Revision as of 03:11, 18 October 2021 by Jianfei Song (Talk | contribs)

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Protein scaffold for rhlA,B,C complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1790
    Illegal XhoI site found at 1799
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 596
    Illegal NgoMIV site found at 911
    Illegal NgoMIV site found at 1226
    Illegal NgoMIV site found at 1541
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 826
    Illegal BsaI site found at 1141
    Illegal BsaI site found at 1456
    Illegal BsaI site found at 1771
    Illegal SapI.rc site found at 455


Source

This study

References

Dueber, J., Wu, G., Malmirchegini, G. et al. Synthetic protein scaffolds provide modular control over metabolic flux. Nat Biotechnol 27, 753–759 (2009). https://doi.org/10.1038/nbt.1557

Du, J., Zhang, A., Hao, J., & Wang, J. (2017). Biosynthesis of di-rhamnolipids and variations of congeners composition in genetically-engineered Escherichia coli. Biotechnology Letters, 39(7), 1041–1048. https://doi.org/10.1007/s10529-017-2333-2

Gong ZJ, Peng YF, Zhang YT, et al. Construction and optimization of Escherichia coli for producing rhamnolipid biosurfactant. Chin J Biotech, 2015, 31(7): 1050–1062.