Coding

Part:BBa_K3861022

Designed by: Mario Delgadillo   Group: iGEM21_Humboldt_Berlin   (2021-10-01)
Revision as of 13:35, 17 October 2021 by Martje1998 (Talk | contribs)


hilA transcriptional regulator

hilA, together with hilC and hilD, are global regulators and control the expression of the Salmonella pathogenicity island I(SPI-1) genes in S. typhimurium on which the SPI-1 T3SS injectisome is encoded (Widmaier et al., 2009). Over 25 genes that are needed for host invasion are located on the SPI-1 (Darwin and Miller, 1999). Also, hilA activates SPI-1 operon mediated by the prg and inv promoters (Bajaj and Lee et al., 1995; Bajaj et al., 1996). hilA controls SPI genes in response to enviromental needs like pH and oxygen levels (Ahmer et al., 1999; Bajaj et al., 1996, Main-Hester et al., 2008).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1635
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1112
    Illegal PstI site found at 1309
    Illegal NgoMIV site found at 151
    Illegal AgeI site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None