Coding

Part:BBa_K3815006

Designed by: Shiotsu Koudai   Group: iGEM21_Kyoto   (2021-10-16)
Revision as of 09:29, 17 October 2021 by Shiotsu (Talk | contribs)


No part name specified with partinfo tag. This part is made of His tag for peptide production. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the targeted protein is CecropinA derived from a moth. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. This sequence got from the moth. In our experiment, we used it to kill the bacteria in the vase water.

Sequence and Features No part name specified with partinfo tag.


[edit]
Categories
Parameters
None