Coding

Part:BBa_K3815002

Designed by: Shiotsu Koudai   Group: iGEM21_Kyoto   (2021-10-15)
Revision as of 08:55, 17 October 2021 by Shiotsu (Talk | contribs)


AANDENYAPHA. mutant SsrA degradation tag

This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein. In this part, the purpose protein is Defencine1 derived from a honey bee. This is antimicrobial peptide that can inhibit the growth of gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None