Part:BBa_P1010

ccdB cell death gene

The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.

P1010 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die and the surviving colonies are the desired result.

Also see P1016, a smaller part containing ccdB but not the inactive ccdA contained in P1010.

BioBrick Construction Plasmids Containing This Part

Most BioBrick plasmids are delivered with the P1010 insert see P1010 Physical DNA for the current list of plasmids that are available in this form. This particular Biobrick with its Plasmid backbone is considered a Construction Plasmid.

Usage and Biology

For more information on how to use this brick, visit its Featured Parts:Cell Death page