Generator

Part:BBa_K3962352:Design

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-13)
Revision as of 10:11, 13 October 2021 by Registry (Talk | contribs)

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Inducible expression of antitoxin CcdA by pBAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Design: pBAD has been intensively investigated and has tight regulation upon downstream genes. It is a suitable promoter for regulating antitoxin genes because pBAD can induce very high levels of CcdA expression to neutralize toxic effects of CcdB.



Source

Source: All sequences of subparts are from iGEM registry.


References