Part:BBa_K3745040
RhlABC + tags + scaffold
This plasmid contains rhlA,B,C as well as tags for each enzyme and scaffold to connect these three enzymes together, and it is the most essential plasmid in our protein scaffold project. We place the ORF of specific tags in front of each enzyme's ORF. These tags could bind with the corresponding domain on the scaffold and form a protein complex that can carry out the three needed reactions to synthesize rhamnolipid.
Referring to "", we adjust the ratio of each domain on the scaffold protein to fine-tune the number of each enzyme and maximize the production efficiency of the reaction.
By connecting rhlA,B,C together, we successfully improved the reaction efficiency of the enzymes that assemble rhamnolipid by maximizing the collision probability of substrates and enzymes as well as minimizing the time interview between each reaction, hence increase rhamnolipid yield.
For the detailed function of each enzyme, see BBa_K3745030
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2281
Illegal BamHI site found at 6640
Illegal XhoI site found at 6649 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2149
Illegal NgoMIV site found at 2554
Illegal NgoMIV site found at 4123
Illegal NgoMIV site found at 5446
Illegal NgoMIV site found at 5761
Illegal NgoMIV site found at 6076
Illegal NgoMIV site found at 6391
Illegal AgeI site found at 3166 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5676
Illegal BsaI site found at 5991
Illegal BsaI site found at 6306
Illegal BsaI site found at 6621
Illegal SapI.rc site found at 4262
Illegal SapI.rc site found at 5305
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