Generator

Part:BBa_K3962342

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-11)
Revision as of 15:32, 11 October 2021 by Registry (Talk | contribs)

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arabinose-mediated inducible expression of toxin CcdB

This construct is composed of L-arabinose-mediate inducible promoter pBAD and toxin CcdB. CcdB/CcdA is a type II toxin/antitoxin system originated from E. coli. In our project, we used this construct to test the toxicity of toxin CcdB, which provided us with more insights to regulate the toxin/antitoxin system for preventing horizontal gene transfer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1902
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1352
    Illegal SapI site found at 961


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Categories
Parameters
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