Composite

Part:BBa_K4040020

Designed by: Tong Yin   Group: iGEM21_NMU_China   (2021-09-27)
Revision as of 11:31, 11 October 2021 by Ytong (Talk | contribs)


Synthetic Receptor GP130-TEV

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1508
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Based on the mechanism of Tango/MESA, we bind TEV-protase to GP130. And when the concentration of IL-6 rises to a certain level, IL-6 binds to mIL-6R (membrane-bound IL-6R), homodimerization of gp130 is induced. A high-affinity functional receptor complex of IL-6, IL-6R and gp130 is formed.  Then, TEV-protase connected behind is activated. TEV-protase binds to TCS and releases transcription factors on the other two receptors through enzyme digestion to initiate the subsequent pathway.  



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