DNA

Part:BBa_K3734001:Design

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-01)
Revision as of 10:03, 9 October 2021 by Shinichi (Talk | contribs) (→‎References)

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CHREBP promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 584
    Illegal BamHI site found at 346
    Illegal XhoI site found at 449
    Illegal XhoI site found at 2640
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3317
    Illegal BsaI site found at 3385
    Illegal BsaI site found at 3632
    Illegal BsaI.rc site found at 452
    Illegal BsaI.rc site found at 475
    Illegal BsaI.rc site found at 2160
    Illegal BsaI.rc site found at 2270
    Illegal SapI.rc site found at 3150


Design Notes

Because it has longer sequences, it require strains with less recombinase to propagation.


Source

Homo species.

References

[1]Jian Meng1, Ming Feng1, Weibing Dong.Identification of HNF-4α as a key transcription factor to promote ChREBP expression in response to glucose.Science reports, 6:23944(2016)