BioScaffold Parts

BioScaffold parts are BioBrick parts that contain restriction enzyme recognition sites that enable their full or partial removal from a BioBrick assembly.

The enzymes that facilitate the removal of BioScaffold parts are defined by what region of the part that they excise:

External Enzymes: Do not cut solely inside the BioBrick Part (extend to Prefix, Suffix, and/or Scar regions)

Internal Enzymes: Cut inside the BioBrick Part (between the Prefix, Suffix, and/or Scar regions)

The classes of α, β, and γ BioScaffold parts are defined for assemblies of the form: <Prefix> part1 <Scar>BioScaffold part<Scar>part2<Suffix>

class α parts: specific enzymes cut outside of scars surrounding the BioScaffold part

class β parts: specific enzymes cut within scars surrounding BioScaffold part

class γ parts: no specific enzymes, internal enzymes cut within the BioScaffold part

mixed parts: contain properties of several classes, name classes involved

Currently, BioScaffold parts and their status must be manually added to the following table.

-?-	Class	Name	Description	External Enzymes	Internal Enzymes
A	α	Part:BBa_J70010	A BioScaffold Part (for offset see Part Design page)	PpiI	MabI, PacI
A	α	Part:BBa_J70012	A BioScaffold Part (Protein Head Remover)	PsrI	MabI, AscI
	α	Part:BBa_J70030	A BioScaffold Part (Protein Tail Remover/Protein Fusions)	PpiI	MabI, PacI
	α	Part:BBa_J70032	A Composite BioScaffold Part (Protein Fusions)	PpiI, PsrI	MabI, (AscI and PacI)
	β	Part:BBa_J70034	A BioScaffold Part (for Library Vector Preparation)	AarI	XmaI
	β	Part:BBa_J70036	A BioScaffold Part (for Library Insert Preparation)	AarI	XmaI