Regulatory
mRNA bindi
Part:BBa_K3805639
Designed by: Yue Tang Group: iGEM21_BNU-China (2021-10-01)
mRNA binding site
Taking proinsulin as an example, we hope to use this switch of mRNA interaction to ensure the normal growth of the strain without expressing the downstream killing system when the expression level of insulin mRNA is normal, and to start the expression of the downstream killing system and kill the deceiver when the mutation at the key site of proinsulin gene leads to the abnormal expression level of proinsulin mRNA. At the same time, we hope to explore the development possibility of mRNA interaction switch, and explore the optimal length ratio and optimal conditions of two mRNA interactions.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |