Composite

Part:BBa_K3722010

Designed by: Yongzhu Li   Group: iGEM21_NWU-CHINA-A   (2021-09-30)
Revision as of 05:25, 30 September 2021 by Registry (Talk | contribs)

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T7_Promoter-RBS-Fre-Linker-SttH-T7_Terminator

Fre is a unique N-terminal tag that efficiently enhanced SttH activity by increasing solubility as well as cofactor supply, the improvement might be caused by an enhanced electron transfer efficiency due to the prox-imity effect of the fusion of Fre and SttH, and greatly improved the yield and productivity of bromination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2384
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1017
    Illegal NgoMIV site found at 1275
    Illegal AgeI site found at 1504
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 480


[edit]
Categories
Parameters
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