Part:BBa_K4033002

ZhuJ

ZhuJ is a CYC2 that promotes the regiospecific C5-C14 second-ring cyclization for non-reduced polyketide chains of the R1128 family.[1]

Biology and Usage

ZhuJ and OKS encodes the aromatase that catalyzes the C5-C14 second-ring closure, which spontaneously leads C2-C15 to form the third-ring, and produce a 2.7-fold increase in flavokermesic acid levels(Fig.a). ZhuJ is proposed to catalyze the condensation between C5 and C14 to yield intermediate SEK4, which is the byproduct of the reaction. After the cyclization of the third-ring (C2-C15) forms spontaneously, flavokermesic acid anthrone forms.[2]

ZhuI and ZhuJ are usually used together for typeⅡPKS reconstitution.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 312
  Illegal NgoMIV site found at 594
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 160
  

Experimental approach

References

[1] Ames, B. D., Lee, M. Y., Moody, C., Zhang, W., Tang, Y., & Tsai, S. C. (2011). Structural and biochemical characterization of ZhuI aromatase/cyclase from the R1128 polyketide pathway. Biochemistry, 50(39), 8392–8406. https://doi.org/10.1021/bi200593m

[2] Frandsen, R.J.N., Khorsand-Jamal, P., Kongstad, K.T. et al. Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans. Sci Rep 8, 12853 (2018). https://doi.org/10.1038/s41598-018-30816-9