Signalling

Part:BBa_K084008:Experience

Designed by: Masahiro Tominaga, Yoshimi Iyama, Kohei Kawasaki   Group: iGEM08_Chiba   (2008-09-18)
Revision as of 09:10, 30 October 2008 by Masahiro (Talk | contribs)

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Applications of BBa_K084008

User Reviews

The iGEM Chiba team conducted tests on this part in the summer of 2008. The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].

One part of the senders cultures were mixed with 1 part of the receiver culture. (Mixed sender culture 1000μL with receiver culture 1000μL.)

Senders strain:XL10Gold,Receiver strain:JW1908.Reaction temparature:30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by BBa_K084012,BBa_K084008,BBa_K084007 respectively. respectively.
Senders strain:XL10Gold,Receiver strain:JW1908.Reaction temparature:37°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by BBa_K084012,BBa_K084008,BBa_K084007 respectively.


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iGEMChiba 2008

The part was tested using this [http://2008.igem.org/Team:Chiba/protocol/phenotype/timedelay protocol].

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UNIQ5cf3dce96ea7d999-partinfo-00000001-QINU UNIQ5cf3dce96ea7d999-partinfo-00000002-QINU