Composite

Part:BBa_K3408009:Design

Designed by: Yunong Li   Group: iGEM20_NAU-CHINA   (2020-10-08)
Revision as of 02:12, 28 October 2020 by Oranjia (Talk | contribs)


PliaG-trigger RNA-B0015-PCⅠ-switch RNA-GFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 239
    Illegal BglII site found at 461
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1190


Design Notes

We wanted to make sure that our toehold switch could work normally by checking if there was GFP in our culture medium.

Source

We designed it with our basic part trigger RNA (BBa_K3408002) and switch RNA (BBa_K3408003).

References