Part:BBa_K3408004

Optimized CⅠ

It is a protein which could suppress P_CⅠ so that our switch RNA couldn't be transcribed without oxygen.

We designed it under the control of P_nar.

We have optimized the CⅠ protein on ThermoFisher SCIENTIFIC (https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria.

                                 Fig.1. Codon quality distribution                                              Fig.2. Optimized codon quality distribution

The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (Sharp, P.M., Li, W.H., Nucleic Acids Res. 15 (3),1987).

                                 Fig.3. Codon quality plot                                                         Fig.4. Optimized codon quality plot

The plots show the quality of the used codon at the indicated codon position.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]