Regulatory

Part:BBa_K3408000

Designed by: Fengzhi Yuan   Group: iGEM20_NAU-CHINA   (2020-10-02)
Revision as of 01:04, 28 October 2020 by Oranjia (Talk | contribs)

promoter nar(fnr regulated)


Pnar is an oxygen-free inducible promoter which can response to the ResD/E two-component regulatory system and the global regulator Nitrate Reductase Regulator (FNR) of Bacillus subtilis.

In our project, we use it to express Phy(ycD) efficiently in the absence of oxygen, and meanwhile express CⅠ repressor protein to inhibit toehold switch-mazF downstream.

According to the paper, Pnar with three levels of strength were designed and applied in E.coli successfully. The expression level of GFP in the control of high-strength promoter was 19.7 higher than the wild-type promoter.

T--NAU-CHINA--basic-parts1.png

Fig.1. The spacer sequence of 15 bp between the -35 and -10 elements of the upstream region of the wild-type nar promoter was randomized(Hwang Hee Jin, et al., BIOTECHNOL BIOFUELS. 2018)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None