Part:BBa_I12007

Modified lambda Prm promoter (OR-3 obliterated)

Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)

Usage and Biology

FIGURE 4. The relative fluorescence of WT (E.coli-wildtype), J23110 (E.coli-pSB1C3-K608010), HrtO (E.coli-pUC-GW-HrtO-GFP) and I12007 (E.coli-pSB1C3-I12007-GFP-pSB1K3-HrtO-cI).
Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm. Relative fluorescence is calculated by the formula below, LB medium as blank. Relative fluorescence is shown in logarithmic axis, 2 to the power of n. As shown in the figure, the expression strength of I12007 is about 8% of HrtO, under the activation of cI (promoter: HrtO), showing 92% of loss during signal transformation from HBS to BAS.

FIGURE 5. Calculating formula.
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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]