Coding

Part:BBa_K3657026

Designed by: Julia Langer   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 22:58, 27 October 2020 by Liza ch (Talk | contribs) (→‎Usage and Biology)


MS2 Coat Protein + SoxS Trancription Activator
This part is a fusion protein consisting of SoxS activator (BBa_K3657024) and MS2 capsid protein (BBa_K3218006).

Usage and Biology

MS2 capsid protein binds specifically to the MS2 RNA hairpin (BBa_K3113006). SoxS is a powerful activator in E. coli.

This construct was used together with a RNA·DNA-DNA triple helix containing an MS2 hairpin (BBa_K3657025). Use of these two parts together allows to attract the SoxS activator to the specific location on a DNA. More information available here: iGEM Heidelberg 2020.

Due to the COVID19 pandemic we were not able to get any empirical evidence about theperformance of this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None