DNA

Part:BBa_K3595014

Designed by: Xinyang Xie   Group: iGEM20_GZ_HFI   (2020-10-27)
Revision as of 13:25, 27 October 2020 by Registry (Talk | contribs)

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DNA that forms a guide RNA to guide cas9 to cut the gene

Cas9 protein in CRISPR-cas9 needs a guide RNA to combine with so that it can cut the certain position on the DNA. In knocking out the argR gene, the guide RNA is transcripted by the loaded DNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None