Coding

Part:BBa_K3657014:Design

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 11:08, 27 October 2020 by Liza ch (Talk | contribs) (→‎References)


Antitermination protein N from Lambda Phage


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The aminoacid sequence was obtained from the Uniprot database (P03045). The nucletide sequence was then generated and codon optimised for E. coli.

Source

Bacteriophage lambda

References

Parks AR, Court C, Lubkowska L, Jin DJ, Kashlev M, Court DL. Bacteriophage λ N protein inhibits transcription slippage by Escherichia coli RNA polymerase. Nucleic Acids Res. 2014 May;42(9):5823-9. doi: 10.1093/nar/gku203. Epub 2014 Apr 7. PMID: 24711367; PMCID: PMC4027172.