RNA

Part:BBa_K3657025

Designed by: Julia Langer   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 00:12, 27 October 2020 by Liza ch (Talk | contribs)


RNA Part of an RNA·DNA-DNA triple helix + MS2-Stemloop

This part consists of a sequence that forms an RNA·DNA-DNA triple helix (DNA part: BBa_K3657023) and a sequence that forms an MS2 hairpin (BBa_K3113006).

Usage and Biology

The MS2 hairpin binds to the MS2 protein (BBa_K3218006). The triple helix forming part binds the DNA sequence specifically. In that way this part should theoretically attract a protein of interest fused to an MS2 protein to a specific region on DNA. Due to COVID19 pandemic we didn't manage to get any empirical evidence that this part functions as described above. More information available here: iGEM Heidelberg 2020


Figure 1: Secondary structure of BBa_K3657025
MS2 hairpin forms a stable structure. The triple helix forming sequence doesn't form any stable structure and does not interfere with the MS2 hairpin. The structure was generated by Geneious software.


Sequence and Features
Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

[edit]
Categories
Parameters
None