Composite

Part:BBa_K3396010

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-25)
Revision as of 13:45, 25 October 2020 by Registry (Talk | contribs)

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GFPnano-FKBP-P2A-Trim21-FRB

CMV->Kozak->Truncated TRIM21->FRB->P2A->FKBP->GFPnano->BGH-pA

This part is designed to demonstrate a small molecule induced target protein degradation with the Predator Pro system. Herein, FRB-FKBP pairs were used to mediate the rapamycin induced trimerization of truncated Trim21, GFP nanobody and target protein EGFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 721


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Categories
Parameters
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