Reporter

Part:BBa_K3466007:Design

Designed by: Isabela Gonzalez   Group: iGEM20_FDR-HB_Peru   (2020-10-24)
Revision as of 22:25, 24 October 2020 by Registry (Talk | contribs)

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T7 -> RBS -> MerR -> PcadA -> RBS -> mRFP -> TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 379
    Illegal AgeI site found at 1152
    Illegal AgeI site found at 1264
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used a T7 promoter to test for better compatibility with the Sigma 70 Master Mix from Arbor Biosciences.


Source

T7 promoter - BBa_K3033000 - BBa_, RBS+MerR - BBa_K1724002, PcadA promoter - BBa_K1724000, RBS - BBa_J34801, mRFP - BBa_I13521, TT - BBa_B0015


References