Composite

Part:BBa_K3440001

Designed by: Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 22:23, 24 October 2020 by JuCo (Talk | contribs) (→‎Characterization)


mCherry under bphR1 promoter

PbphR1(BBa_K1155001)-RBS(BBa_B0034)-mCherry(BBa_J06504)-DT(BBa_B0015)

Usage and Biology

Produces mCherry under bphR1 promoter, which can be activated by bphR2:Polychlorinated biphenyl.

Characterization

Due to the pandemics, we haven’t been able to use biobricks to create the iGEM Stockholm 2020 parts. Those parts were ordered as gene blocks from Integrated DNA Technologies Inc.. As a result, the sequences of the biobricks used are the same, but the scars between biobricks might differ, as well as the final size of the part.

Western Blot for BBa_K34440001 and BBa_K3440002

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 156
    Illegal BamHI site found at 239
    Illegal XhoI site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 139


[edit]
Categories
//cds/reporter/rfp
//chassis/prokaryote/ecoli
Parameters
colorred
emission615
excitation560
originbphR1 promoter from Pseudomonas pseudoalcaligenes KF707 and GFP from Anaplasma marginale