Reporter

Part:BBa_K3466003

Designed by: Marry Xuan, Isabela Gonzalez   Group: iGEM20_FDR-HB_Peru   (2020-10-24)
Revision as of 22:08, 24 October 2020 by Registry (Talk | contribs)

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CP -> RBS -> MerR -> PcadA -> RBS -> mRFP -> TT

This plasmid is an alternate version of construct 1 with the addition of BamHI and BgIII restriction sites surrounding the RBS. These restriction sites allow us to cut and insert the single RFP part (BBa_K3466006) into the plasmid. This is because in our previous experiment, having more RFP genes may have increased the intensity and the rate of expression of RFP. Therefore, we want to test whether more RFP genes lead to better RFP expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 293
    Illegal AgeI site found at 395
    Illegal AgeI site found at 1168
    Illegal AgeI site found at 1280
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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