Composite

Part:BBa_K3440018

Designed by: Julie Cordier, Victoria Muliadi   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 15:17, 24 October 2020 by JuCo (Talk | contribs)


PCB detection

Pconst(BBa_J23100)-RBS(BBa_B0034)-bphR2(BBa_K1413021)-Myc(BBa_K823036)-DT(BBa_B0015)-Pbphr1(BBa_K1155001)-RBS(BBa_B0034)-LuxI(BBa_C0061)

Usage and Biology

This is the final construct to detect a PCB and consequently produce a quorum sensing molecule. bphR2 is produced constitutively and can bind to a PCB. The complex PCB:bphR2 can activate bphR1 promoter, under which LuxI is placed. LuxI produces the 3OC6-HSL quorum sensing molecule.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 996
    Illegal BglII site found at 2108
    Illegal BamHI site found at 1301
    Illegal BamHI site found at 1384
    Illegal XhoI site found at 1191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 320
    Illegal SapI.rc site found at 1284


[edit]
Categories
Parameters
None