Part:BBa_K3506008

Linker peptide KKKKS

This part can be used as a linker for protein fusion in yeast. Its nucleotide sequence is aagaagaagaagtcc. Its codon has been optimized based on Codon Usage frequency in highly expressed genes of yeast (Figure 1). We designed this part to fuse the first 124 amino acids of Clb2 and Cas9.

Usage

This part can be used in the fusion of two proteins in yeast. To fuse two proteins, one can add this part between his sequences by designing primers containing the sequence of this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1]Hendrickson, C., Meyn, M. A., 3rd, Morabito, L., & Holloway, S. L. (2001). The KEN box regulates Clb2 proteolysis in G1 and at the metaphase-to-anaphase transition. Current biology : CB, 11(22), 1781–1787.

[2]Sharp, P. M., Tuohy, T. M., & Mosurski, K. R. (1986). Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes. Nucleic acids research, 14(13), 5125–5143.