Part:BBa_K3505040

pDGB1 alpha BACKBONE for Bacterial GoldenBraid Cloning

This backbone combined with the proper insert BBa_K3505041 results in the comlpete Vectors BBa_K3505008BBa_K3505009

Fig.1: a1R plasmid Features and Restiction Sites.

Fig.1: a2 plasmid Features and Restiction Sites.

Experimental Use and Experience

The following parts were cloned in these 2 vectors BBa_K3505025 BBa_K3505026 BBa_K3505027 BBa_K3505028 BBa_K3505029 BBa_K3505030 BBa_K3505031 BBa_K3505032 BBa_K3505033 BBa_K3505034 BBa_K3505035 BBa_K3505038 BBa_K3505039

pDGB1 a1R verification with no insert

Fig.3: pDGB1 a1R Digested with BamHI. Expected bands 2847, 385, 239 and pDGB1 a2 Digested with HindIII. Expected bands 2847, 416, 206

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1274
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

• [1]Alejandro Sarrion-Perdigones, Marta Vazquez-Vilar, Jorge Palací, Bas Castelijns, Javier Forment, Peio Ziarsolo, José Blanca, Antonio Granell, Diego Orzaez (2013). “GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology.” Plant Physiology , 162 (3) 1618-1631; DOI: 10.1104/pp.113.217661
• [2]Esteban Martínez-García, Angel Goñi-Moreno, Bryan Bartley, James McLaughlin, Lucas Sánchez-Sampedro, Héctor Pascual del Pozo, Clara Prieto Hernández, Ada Serena Marletta, Davide De Lucrezia, Guzmán Sánchez-Fernández, Sofía Fraile, Víctor de Lorenzo, SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts, Nucleic Acids Research, Volume 48, Issue D1, 08 January 2020, Pages D1164–D1170, https://doi.org/10.1093/nar/gkz1024