Coding
Part:BBa_K3505041
Designed by: Venetios Michelioudakis Group: iGEM20_Thessaly (2020-10-23)
LacZa for Bacterial GoldenBraid plasmids
This LacZa moudule is the cargo of the Bacterial Golden Braid Plasmid Collection
- pUPD2 backbone BBa_K3505043+ LacZa pUPD2 = BBa_K3505007
- alpha backbone BBa_K3505040+ LacZa α1R = BBa_K3505008
- alpha backbone BBa_K3505040+ LacZa α2 = BBa_K3505009
- omega backbone BBa_K3505042+ LacZa Ω1 = BBa_K3505010
- omega backbone BBa_K3505042+ LacZa Ω2 = BBa_K3505011
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 366
Illegal PstI site found at 405 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 366
Illegal BamHI site found at 387 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
Illegal BsaI.rc site found at 616
[edit]
Categories
Parameters
//awards/part_collection
//plasmid/construction
//plasmid/expression
//plasmid/construction
//plasmid/expression
None |