Coding

Part:BBa_K3505041

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-23)
Revision as of 09:50, 24 October 2020 by Venetios (Talk | contribs)


LacZa for Bacterial GoldenBraid plasmids This LacZa moudule is the cargo of the Bacterial Golden Braid Plasmid Collection

Fig.1: The LacZa module flanking sites for every GB pLamid type
File:T--Thessaly--Goldenbraidgrammar.jpeg
Fig.1: The GoldenBraid Garmmar

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 366
    Illegal PstI site found at 405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 366
    Illegal BamHI site found at 387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 616
[edit]
Categories
//awards/part_collection
//plasmid/construction
//plasmid/expression
Parameters
None