DNA

Part:BBa_K3697003

Designed by: Christopher Neimeth   Group: iGEM20_Stanford   (2020-10-22)
Revision as of 23:27, 23 October 2020 by Cneimeth (Talk | contribs)


Homology Arms for KanR integration in B. Subtilis

This part is derived from a portion of the pOpen Yeast plasmid between the AarI and MspA1I cut sites. More information about pOpen Yeast and how to get it through Stanford Free Genes can be found on their website and at this link: https://stanford.freegenes.org/products/popen_build. This sequence was then divided into two homology arms (homology arm 1 and homology arm 2 as marked in the annotations for the part). When incorporated into the B. subtilis genome, these two sequences (homology arm 1 and homology arm 2) become the two homology arms needed to trigger B. subtilis' natural process of recombination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//dna
//function/recombination
Parameters
biology
target