Composite

Part:BBa_K3560006

Designed by: ShiDi Xiao   Group: iGEM20_XHD-Wuhan-China   (2020-10-23)
Revision as of 09:28, 23 October 2020 by Registry (Talk | contribs)

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PGroES-RiboJ-DrRBS-gcd

RiboJ is a self-cleaving ribozyme that can remove the UTR sequence at the upstream 5'end. RiboJ has 75 nt, which comprises the sTRSV-ribozyme which is used to cut the 5'-UTR sequence in the promoter with an additional 23-nt hairpin immediately downstream to help expose the RBS. plasmid contenting PGroES-Riboj-RBS-gcd gene circuit was transformed into Deinococcus radiodurans. Enhance the expression of downstream gcd genes, increase the content of GDH, and promote the dissolution of phosphate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal AgeI site found at 1880
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None