RNA

Part:BBa_K3408002

Designed by: Fengzhi Yuan   Group: iGEM20_NAU-CHINA   (2020-10-03)
Revision as of 04:38, 22 October 2020 by NJAU-Chappie (Talk | contribs)

Trigger RNA(of toehold)


We would use the trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide.

We modifed our trigger RNA with some bps in the 5’ terminal to increase its stability.

T--NAU-CHINA--basic-parts2.png Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None