Coding
HNMT

Part:BBa_K3647777

Designed by: Natalia Savinkova, Ida Berghten, Astrid Welin, Thalia Rodriquez, Osamudiamen Iyere, Frida Haugskott, Oliver Hild Walett   Group: iGEM20_Linkoping   (2020-08-20)
Revision as of 08:48, 18 October 2020 by Nataliasavinkova (Talk | contribs)

Histamine N-methyltransferase (N-term. 6XHIS, TEV)


For information and characterization of this part please see: BBa_K3647666

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 40
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 394
    Illegal BsaI.rc site found at 778

General information

Histamine N-methyltransferase is one of the two enzymes involved in the metabolism of histamine in mammals, this is known as the N(tau)-methylation pathway. The second pathway is oxidative deamination via diamine oxidase (DAO). This protein is encoded by the gene HNMT and it can be found in the cytosol intracellular fluid. HNMT is expressed in many organs like liver, kidney and brain, being found in most of the body tissues, except for serum. (Yoshikawa, 2019)

Funtion of HNMT is catalyzation of histamine methylation in the presence of S-adenosylmethionine (SAM-e) yielding N-methylhistamine. Histamine (HA) is inactivated by using S-adenosylmethionine (SAM-e) as a methyl donor. This occurs, as an example, during the termination process of neurotransmission actions of HA in the mammalian central nervous system. (Heidari, 2015) (Yoshikawa, 2019)



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