Composite

Part:BBa_K3332055

Designed by: Yangqi Deng   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 21:14, 17 October 2020 by Registry (Talk | contribs)

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J23100-RBS-GOX-BrkA-terminator

We anchored GOX protein onto membranes through BrkA to catalyze the reaction of degradating glyphosate to form glyoxalic acid and AMPA. We use K880005 to construct the expression system and anchor GOX on the surface of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 1304
    Illegal NgoMIV site found at 1733
    Illegal NgoMIV site found at 2369
    Illegal AgeI site found at 644
    Illegal AgeI site found at 721
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2337


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